What is MS2 protein?
MS2 tagging is a technique based upon the natural interaction of the MS2 bacteriophage coat protein with a stem-loop structure from the phage genome, which is used for biochemical purification of RNA-protein complexes and partnered to GFP for detection of RNA in living cells.
What is MS2 in simple terms?
Escherichia virus MS2 is an icosahedral virus having single-stranded, positive-sense RNA virus. It infects Escherichia coli bacteria and other different members of the Enterobacteriaceae. MS2 is a part of closely related bacterial viruses such as Bacteriophage Qβ, bacteriophage f2, GA, and R17.
What is MS2 organism?
MS2 is a bacteriophage that infects “male” Eschericia coli. Male E. coli are bacterial cells capable of passing a portion of their genetic material (typically in plasmid form) to other bacterial cells through a structure called a pilus.
What is the capsid of bacteriophage?
The protein shell that encloses the nucleic acid genome of phages along with genomes of viruses in general. The capsid is sometimes called a capsule instead. The capsids of most phages takes on an icosahedral shape which is either symmetrical or elongated. …
How does MS2 MCP work?
The MS2-MCP system allows imaging multiple steps of the mRNA life cycle with high temporal and spatial resolution. However for short-lived mRNAs, the tight binding of the MS2 coat protein (MCP) to the MS2 binding sites (MBS) protects the RNA from being efficiently degraded, confounding the study of mRNA regulation.
What is MS2 internal control?
MS2 Bacteriophage can be used as an internal control in nucleic acid-based amplification assays to monitor reverse-transcription and PCR inhibition. Controls should be run using the same protocols as those used to amplify extracted clinical specimens.
What does MS2 affect?
MS2 infects enteric bacteria carrying the fertility (F) factor, a plasmid that allows cells to serve as DNA donors in bacterial conjugation. Genes on the F plasmid lead to the production of an F pilus, which serves as the viral receptor.
Is MS2 a virus?
Bacteriophage MS2 (Emesvirus zinderi), commonly called MS2, is an icosahedral, positive-sense single-stranded RNA virus that infects the bacterium Escherichia coli and other members of the Enterobacteriaceae.