What are the factors affecting enzyme reaction?
The six factors are: (1) Concentration of Enzyme (2) Concentration of Substrate (3) Effect of Temperature (4) Effect of pH (5) Effect of Product Concentration and (6) Effect of Activators. The contact between the enzyme and substrate is the most essential pre-requisite for enzyme activity.
What are the 4 factors that affect enzymatic function?
Factors Affecting Enzyme Activity
- Factor 1: Concentration of Enzyme.
- Factor 2: Concentration of Substrate.
- Factor 3: Effect of Temperature.
- Factor 4: Effect of pH.
- Factor 5: Effect of Activators.
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What factors affect enzyme activity lab?
The main factors which will affect the activity of an enzyme on a given substrate are:
- Temperature (use water baths to minimise fluctuations)
- pH (acidic or alkaline solutions)
- Substrate concentration (choose range to avoid saturation)
- Presence of inhibitor (type of inhibitor will be enzyme-specific)
Are enzymes reaction specific?
Enzymes are highly specific both in the reactions that they catalyze and in their choice of reactants, which are called substrates. An enzyme usually catalyzes a single chemical reaction or a set of closely related reactions.
What are the mechanism of enzyme action?
An enzyme attracts substrates to its active site, catalyzes the chemical reaction by which products are formed, and then allows the products to dissociate (separate from the enzyme surface). The combination formed by an enzyme and its substrates is called the enzyme–substrate complex.
Why do enzymes bind only?
Enzymes are highly selective catalysts, meaning that each enzyme only speeds up a specific reaction. [What Is Chemistry?] The molecules that an enzyme works with are called substrates. In the induced-fit model, the active site and substrate don’t fit perfectly together; instead, they both alter their shape to connect.
What factors affect catalase activity?
The rate at which an enzyme works is influenced by several factors including the concentration of substrate (hydrogen peroxide in the case of catalase), temperature, pH, salt concentration and the presence of inhibitors or activators. Every enzyme has an optimal range for each of these factors.
What three factors affect enzymes?
Enzyme activity can be affected by a variety of factors, such as temperature, pH, and concentration. Enzymes work best within specific temperature and pH ranges, and sub-optimal conditions can cause an enzyme to lose its ability to bind to a substrate.
Why are enzymes very specific?
Enzymes are specific to substrates as they have an active site which only allow certain substrates to bind to the active site. This is due to the shape of the active site and any other substrates cannot bind to the active site. there is a model which is well known in the biology field of the lock and key model.