How much media does a T75 flask have?
Transfer the desired amount of cell suspension to new plates or flasks and add the appropriate amount of media to complete volume. (T75 flask holds 10ml total volume, T25 holds 5ml, and P100 plate holds 8ml).
How much liquid goes in a 6 well plate?
Growth Area of Tissue Culture Plates and Dishes
| Growth Area (cm2) | Maximum Volume (ml) | |
|---|---|---|
| 6 well | 9.5 | 16.8 |
| 12 well | 3.8 | 6.9 |
| 24 well | 1.9 | 3.4 |
| 48 well | 0.95 | 1.6 |
How much is a medium in 12 well?
Useful information for various sizes of cell culture dishes and flasks
| Catalog No. | Growth medium (mL). Approx. volume | |
|---|---|---|
| Dishes | ||
| 12-well | 150628 | 1 to 2 |
| 24-well | 142475 | 0.5 to 1.0 |
| 48-well | 150687 | 0.2 to 0.4 |
How many cells are in a T75 flask?
Each T75 flask can yield ~ 1.2E+6 cells.
What does T25 flask mean?
T25 flasks for suspension culture of non-adherent cells Made with high quality, optically clear virgin polystyrene, the Thermo Scientific™ Nunc™ Non-treated Flasks, with high level sterility assurance, are ideal for suspension culture of non-adherent cells. Neck. Surface. Description. Cap.
What is the diameter of 6 well plate?
6 Well Macroplates Manufactured from crystal clear and heat-resistant polystyrene for optimal analysis by microscopy With 35 mm diameter per well, it is a practical and space-saving alternative to six standard petri dishes Numeric coding allows easier identification of the wells Volume/well: 16 ml With…
How many mL is a 96 well plate?
CulturPlate Microplates
| Well format | 24-well | 96-well |
|---|---|---|
| #columns | 6 | 12 |
| Well volume | 2.39 mL | 400 µL |
| Recommended working volume | 0.5-2.39 mL | 80 µL-350 µL |
| Height (mm) | 18.70 | 14.60 |
What’s the capacity of a 24 well plate?
The XBLOK2412 is an SBS-format 24-well 12mL polypropylene plate with the designed purpose of automating cell culture and genomic extractions. The XBLOK2412 has the largest capacity of any 24-well plates on the market to date (12mL, recommended max working volume of 10.5mL) and when used in tandem… Related Products: 24 Deep Well Plates
How much media to use for 96 well plates?
We use 500uL of media for up to 3 total days in culture without media changes in 24-well plates using adherent mouse insulinoma cells. Works really well. For 96-well plates, we use either 100uL of 150uL for culturing the same mouse insulinoma cells, depending on the preference of the operator.
How big are deep well plates at Thomas scientific?
Well Plates Well dimensions: 22 dia. x 18 deep mm; thickness: 1.52 mm Total volume per well: 6.0 mL; Growth area per well: 3.8 cm 2 Model 6902A05 plates are individually wrapped in a case of 50; Model 6902A07 plates are bulk packaged in bags of six in a case of 36. 24 Well …
Which is the largest deep well plate on the market?
The XBLOK2412 has the largest capacity of any 24-well plates on the market to date (12mL, recommended max working volume of 10.5mL) and when used in tandem… Related Products: 24 Deep Well Plates