What are the inhibitors of glycolysis?

What are the inhibitors of glycolysis?

Most of the reported glycolysis inhibitors are summarized (Table 1 and Figure 1). The enzymes targeted include hexokinase (HK), phosphofructokinase (PFK), pyruvate kinase (PK), lactate dehydrogenase (LDH), and pyruvate dehydrogenase kinase (PDK).

Are there drugs that stop glycolysis?

Lonidamine. This compound is a derivative of indazole-3-carboxylic acid, and has been known for a long time to inhibit aerobic glycolysis in cancer cells (Floridi et al., 1981). In cell culture, lonidamine decreases oxygen consumption in both normal and neoplastic cells.

What enzyme is inhibited by iodoacetate and arsenate?

Iodoacetamide (IAA) and iodoacetate (IA) have frequently been used to inhibit glycolysis, since these compounds are known for their ability to irreversibly inhibit the glycolytic enzyme glyceraldehyde-3-phosphate dehydrogenase (GAPDH).

How can glycolysis be prevented?

To minimize glycolysis, one should place the sample tube immediately in an ice-water slurry, and plasma should be separated from the cells within 30 min. If that cannot be achieved, a tube containing a rapidly effective glycolysis inhibitor, such as citrate buffer, should be used for collecting the sample.

What enzyme is inhibited by arsenate?

pyruvate dehydrogenase
Arsenic interferes with cellular longevity by allosteric inhibition of an essential metabolic enzyme pyruvate dehydrogenase (PDH) complex, which catalyzes the oxidation of pyruvate to acetyl-CoA by NAD+. With the enzyme inhibited, the energy system of the cell is disrupted resulting in a cellular apoptosis episode.

What are the three major phases of glycolysis?

Stages of Glycolysis. The glycolytic pathway can be divided into three stages: (1) glucose is trapped and destabilized; (2) two interconvertible three-carbon molecules are generated by cleavage of six-carbon fructose; and (3) ATP is generated.