What is an Alamar blue assay?

What is an Alamar blue assay?

Overview. alamarBlue is a cell viability assay reagent which contains the cell permeable, non-toxic, and weakly fluorescent blue indicator dye called resazurin. alamarBlue quantitatively measures proliferation in human, animal, bacterial, fungal, and mycobacterial cells.

How does the Alamar blue assay work?

AlamarBlue Cell Viability Reagent quantitatively measures the proliferation of mammalian cell lines, bacteria and fungi. The dye incorporates an oxidation-reduction (REDOX) indicator that both fluoresces and change color in response to the chemical reduction due to cell growth.

What is Alamar?

Alamar Blue Cell Viability Assay (6 Citations) AlamarBlue Cell Viability Assay reagent quantitatively measures the proliferation of mammalian cell lines, bacteria and fungi. In alamarBlue assay the growing cells cause a chemical reduction of the alamarBlue dye from non-fluorescent blue to red fluorescent.

How can I reduce my blue Alamar?

Alamar Blue, therefore, can be reduced by NADPH (Eo = 320 mV), FADH (Eo = 220 mV), FMNH (Eo = 210 mV), NADH (Eo = 320 mV), as well as the cytochromes (Eo = 290 mV to +80 mV). As the indicator dye accepts electrons, it changes from the oxidized, non-fluorescent, blue state to the reduced, fluorescent, pink state [9].

Is Alamar Blue toxic?

Resazurin, or alamarBlue®, is generally nontoxic during typical, manufacturer-recommended assay incubation times of a few hours (Fields and Lancaster 1993; Zhang et al. 2004).

Is Alamar blue light sensitive?

alamarBlue® should be stored in the dark, since the compound is light sensitive (Table 2). The product may be stored for 12 months at room temperature.

How is the Alamar Blue cell proliferation assay used?

alamarBlue is a cell proliferation assay that provides a rapid, sensitive and economical way to quantitatively measure cell proliferation and cytotoxicity in various human and animal cell lines. It can also be used to study mycobacteria, bacteria and fungi.

How is alamarblue used in the treatment of pancreatic cancer?

AlamarBlue® used to evaluate murine pancreatic acinar cell viability in the presence of stimuli following in vitro culture. Analysis of viability of pancreatic acinar cells in culture. Cell viability under the different treatments applied was analyzed studying AlamarBlue reduction, as described in “Material and methods”.

How is alamarblue used to assess HMSC viability?

AlamarBlue ( BUF012B) used to assess hMSC viability in different scaffolds in vitro by FLISA. Cell viability and proliferation results on PEVAL/PDVAL/β-TCP hybrid material scaffolds containing 50 and 70 wt% of β-TCP contents and the PEVAL/PDVAL blend (reference during 1, 2, and 3 days).

How is alamerblue used to treat lung cancer?

AlamerBlue® used for the assessment of tumor slice viability in vitro using fluorescence readout. FAK inhibition reduces cell viability in human lung tumor tissue but not normal lung tissue ex vivo.