What is M13 DNA?
M13 is one of the Ff phages (fd and f1 are others), a member of the family filamentous bacteriophage (inovirus). M13 plasmids are used for many recombinant DNA processes, and the virus has also been used for phage display, directed evolution, nanostructures and nanotechnology applications.
What is the genetic material of M13 bacteriophage?
Complete answer: M13 is a filamentous bacteriophage composed of circular single-stranded DNA (ssDNA) which is 6407 nucleotides long encapsulated in approximately 2700 copies of the major coat protein, and capped with 5 copies of two different minor coat proteins on the ends.
Is M13 phage single-stranded?
M13 is a filamentous bacteriophage which infects E. The M13 genome has the following characteristics: Circular single-stranded DNA. 6400 base pairs long.
Is M13 a lytic or lysogenic phage?
Phages are divided into three main classes based on their production and generation: lytic phages such as T4; temperate phages like Lambda; and lysogenic phages such as M13. M13 is a filamentous phage that converts the host cell into a generation factory without lytic disruption.
Is M13 a temperate phage?
Phage that are capable of both a lytic and lysogenic pathway are called temperate phage. P1 and λ are temperate phage. M13 is unusu- al because phage continually exit from a bacterium without killing it. For this reason, M13 is not considered to have a true lysogenic state and is not a temperate phage.
What is M13 phage vector?
M13 cloning vectors have been developed for the selection of DNA sequences capable of directing initiation of DNA synthesis on single-stranded templates. These vectors are derived from viable M13 mutants containing large deletions in the region of the complementary strand origin.
Which phage is a female specific phage?
The female-specific phages, ϕI, ϕII, W31 and H (but not T3 and T7) show a low efficiency of plating on all F-minus strains of Escherichia coli K12 except for the thr leu thi mutants descended from strain Y53.
What is lambda phage vector?
Enterobacteria phage λ (lambda phage, coliphage λ) is a bacterial virus, or bacteriophage, that infects the bacterial species Escherichia coli. Lambda phage consists of a virus particle including a head (also known as a capsid), tail and tail fibers. The head contains the phage’s double-stranded circular DNA genome.
Is lambda phage a plasmid?
Enterobacteria phage λ (lambda phage, coliphage λ, officially Escherichia virus Lambda) is a bacterial virus, or bacteriophage, that infects the bacterial species Escherichia coli (E. coli)….Lambda phage.
| Escherichia virus Lambda | |
|---|---|
| Genus: | Lambdavirus |
| Species: | Escherichia virus Lambda |
Is phage a vector?
Phage vectors consist of an essentially complete phage genome, often M13 phage, into which is inserted DNA encoding the protein or peptide of interest (Figure 1). The remaining genes of the phage vector or helper phage encode other proteins.
Why is it called lambda phage?
Enterobacteria phage λ (lambda phage, coliphage λ, officially Escherichia virus Lambda) is a bacterial virus, or bacteriophage, that infects the bacterial species Escherichia coli (E. coli). It was discovered by Esther Lederberg in 1950….Lambda phage.
| Escherichia virus Lambda | |
|---|---|
| Genus: | Lambdavirus |
| Species: | Escherichia virus Lambda |
Which is largest capacity vectors?
The P1-derived artificial chromosomes are DNA constructs derived from the DNA of P1 bacteriophage and BAC. They can carry large amounts (about 100–300 kb) of other sequences for a variety of bioengineering purposes. It is one type of vector used to clone DNA fragments (100- to 300-kb insert size; average, 150 kb) in E.