What is the Vmax of acid phosphatase?
The purified phosphatase had maximum catalytic activity at pH 5.5, and displayed optimal activity at 50 °C. The phosphatase had a Km of 0.24 mM and a Vmax of 2.76 μmol/min/mg with PLP.
What does acid phosphatase do in wheat germ?
Acid phosphatase (APase) non-specifically catalyzes the hydrolysis of monoesters and anhydrides of phosphoric acid to produce inorganic phosphate. It is used to study the production, transport, and recycling of phosphate and the metabolic and energy transduction processes of the cell.
What is the Vmax of alkaline phosphatase?
The Km and Vmax values were 0.5 x 10-3 M and 20 x 10-6 M/min, respectively. Inhibition studies showed that ALP activity was competitively inhibited by 0.67 mM sodium hydrogen orthophosphate (NaH2PO4) and the inhibition constant (Ki) was 0.9 x 10-3 M.
What do the values Vmax and Km represent in terms of Characterising the acid phosphatase being used?
Under the conditions of this study, the Km refers to the apparent Km for the enzyme activity, or the concentration of substrate at which activity is one half the maximal velocity and Vmax refers to the apparent maximal velocity for enzyme activity, which is the maximum rate of P- hydrolysis.
What activates acid phosphatase?
Acid phosphatase catalyzes the following reaction at an optimal acidic pH (below 7): Orthophosphoric monoester + H2O → alcohol + H3PO. Phosphatase enzymes are also used by soil microorganisms to access organically bound phosphate nutrients.
Where is wheat germ acid phosphatase found?
Commercial wheat germ acid phosphatase (WGAP) is extracted from the germ/embryo of wheat plants and is commonly used in vitro in many biochemistry laboratories.
What is the Km value of alkaline phosphatase?
8.4 x 10
Alkaline phosphatase has a Km of 8.4 x 10−4.
What is the function of ALP?
ALP is an enzyme found in your bloodstream. It helps break down proteins in the body and exists in different forms, depending on where it originates. Your liver is one of the main sources of ALP, but some is also made in your bones, intestines, pancreas, and kidneys.
Why is it important that enzymes are reusable?
Enzymes are reusable. Once an enzyme binds to a substrate and catalyzes the reaction, the enzyme is released, unchanged, and can be used for another reaction. This means that for each reaction, there does not need to be a 1:1 ratio between enzyme and substrate molecules.
What happens to the enzymes at high temperatures?
Higher temperatures disrupt the shape of the active site, which will reduce its activity, or prevent it from working. The enzyme will have been denatured . High temperatures will break these forces. The enzyme, including its active site, will change shape and the substrate no longer fit.