What type of DNA mutation is caused by ethidium bromide?
Ethidium bromide, which intercalates between base pairs in DNA, induces cytoplasmic respiratory “petite” mutations in yeast in the absence of growth (Slonimiski et al., 1968). This effect is specific for this intercalating agent and results in a shortening of mitochondrial DNA (Goldring et al., 1970).
How does ethidium bromide cause mutations?
Article Platelet mediated transformation of human mitochondrial DNA-… Mutagenic intercalating agents (e.g., ethidium bromide) can cause insertions during DNA replication. Loss of intercalating agent can result in deletion.
Which type of mutations are caused by ethidium bromide a intercalating dye?
Which techniques ethidium bromide is used?
Although ethidium bromide is routinely used to stain DNA in gels, ethidium bromide has also been used to detect protein–DNA complexes in band shift assays and to observe single DNA molecules during gel electrophoresis.
How do you wash off ethidium bromide?
Charcoal Filtration: Filtering the aqueous EtBr waste solutions (free of other contaminants) through a bed of activated charcoal is a relatively simple and effective method for removal of EtBr. The filtrate may be poured down the drain.
How does the intercalation of ethidium bromide affect DNA?
The intercalation may cause mutation of DNA. Ethidium bromide is thought to act as a mutagen because it intercalates double-stranded DNA (i.e. inserts itself between the strands), deforming the DNA. This could affect DNA biological processes, like DNA replication and transcription.
How is ethidium bromide used in life science?
Ethidium Bromide Ethidium Bromide is an intercalating agent which resembles a DNA base pair. Due to its unique structure, it can easily intercalate into DNA strand. Therefore, it is commonly used as nucleic acid fluorescent tag in various techniques of the life science field.
How is ethidium bromide similar to a base pair?
Ethidium bromide is a relatively large, flat basic molecule that somewhat resembles a DNA base pair. Because of its chemical structure, it can easily insert into a DNA strand, making it handy for experimental purposes, but dangerous to handle by the investigator.
What is the molecular weight of ethobromide?
Known also as ethobromide, homidium bromide, and dromilac, ethidium bromide has a molecular weight of 394.31 and the rather complex chemical name of 3,8-diamino-5-ethyl-6-phenylphenanthridinium bromide.