What is BsaI?
Golden Gate cloning or Golden Gate assembly is a molecular cloning method that allows a researcher to simultaneously and directionally assemble multiple DNA fragments into a single piece using Type IIs restriction enzymes and T4 DNA ligase. Most commonly used Type IIS enzymes include BsaI, BsmBI, and BbsI.
What is CutSmart buffer?
Our CutSmart Buffer incorporates BSA to enable even more enzymes to cut in a single buffer (>200 enzymes). This allows for enhanced ease of use especially when doing double digests. In addition, it eliminates the extra tube of BSA and means one less thing to think about when setting up restriction enzyme digests.
How does Golden Gate cloning work?
The principle of Golden Gate cloning consists of using a type IIS restriction enzyme and ligase in a restriction-ligation to assemble several DNA fragments in a defined linear order in a vector in a single step (Fig. 2A). Type IIS restriction enzymes cleave DNA outside of their DNA recognition site sequence.
Does CutSmart have BSA?
Since CutSmart Buffer includes BSA, there are also fewer tubes and pipetting steps to worry about. Additionally, many DNA modifying enzymes are 100% active in CutSmart Buffer, eliminating the need for subsequent purification.