What is Satellitism in microbiology?
[sat´ĕ-li-tizm] the phenomenon in which certain bacterial species grow more vigorously in the immediate vicinity of colonies of other unrelated species, owing to the production of an essential metabolite by the latter species.
Which organism exhibits Satellitism with Staphylococcus aureus?
However, a related species, H. ovis, is known to exhibit satellitism around S. aureus, as well as pyridoxal dependence, in a manner similar to that of so-called nutritionally variant streptococci (23).
What is meant by Satelitism?
Medical Definition of satellitism : the growth of bacteria of one type in culture about colonies of another type that supply needed micronutrients or growth factors.
What is the satellite phenomenon with respect to Haemophilus?
Staphylococcus aureus produce NAD as a metabolic byproduct when grow in a culture media containing blood . Therefore, Haemophilus spp may grow on sheep blood agar very close to the colonies of Staphylococcus aureus (as it produces NAD-factor V); this phenomenon is known as satelliting.
Which bacteria is known for Satelliting?
The NAD diffuses into the surrounding medium and stimulates growth of Haemophilus in the vicinity of the staphylococcus colony. This is known as satelliting.
What is platelet Satellitism?
Platelet satellitism (PS) is a rare phenomenon observed in blood smears obtained from blood anticoagulated with EDTA. It is characterised by platelet rosetting around polymorphonuclear neutrophils and in rare cases around other blood cells.
Which bacteria can be determined by satellite method?
Background — Haemophilus influenzae can grow on blood agar media with Staphylococcus aureus which can provide factor V as it is called “Satellite phenomenon”.
What is Camp test in microbiology?
The CAMP test (Christie–Atkins–Munch-Peterson) is a test to identify group B β-hemolytic streptococci (Streptococcus agalactiae) based on their formation of a substance (CAMP factor) that enlarges the area of hemolysis formed by the β-hemolysin elaborated from Staphylococcus aureus.
Is influenza Gram positive or negative?
The epidemiology of human influenza and bacterial coinfection demonstrates an overwhelming preponderance of infections with gram-positive bacteria.
How can you tell if an organism has satellitism?
If satellites colonies are present on both blood and nutrient agar plates than the organism is probably a Haemophilus species that requires only factor V, such as H. parainfluenzae. Note: Very occasionally satellitism is shown by strains of Neisseria, Streptococcus species and diphtheroids.
What is the procedure for the satellitism test?
Procedure of Satellitism test to identify Haemophilus influenzae. Mix a loopful of suspected colonies of Haemophilus colonies in about 2 ml of sterile physiological saline (or sterile peptone water). Make sure none of the Chocolate agar medium is transferred.
How does the satellitism test for Haemophilus influenzae work?
Satellitism and satellitism test procedure to identify Haemophilus influenzae. Most strains of Haemophilus spp does not grow on 5% Sheep Blood Agar, which contains hemin (factor X) but lacks NAD (factor V). Staphylococcus aureus produce NAD as a metabolic byproduct when grow in a culture media containing blood .
Which is the first free living organism to show satellitism?
Note: Very occasionally satellitism is shown by strains of Neisseria, Streptococcus species, and diphtheroids. Haemophilus influenzae is the first free-living organism to have its genome (complete genetic code) sequenced.